Abstract: Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an objective method for qualitative protein detection. The objective of this study was to utilize SDS-PAGE in the analysis of proteinuria in canine systemic inflammatory response syndrome (SIRS) and to evaluate its potential as a prognostic indicator by comparison with clinicopathologic parameters and Acute Patient Physiologic and Laboratory Evaluation scores (APPLEfast and APPLEfull). Thirty-one dogs with renal proteinuria were categorized into two groups: SIRS (n=10) and non-SIRS (n=21), and electropherograms were generated following urine SDS-PAGE. The molecules were classified into low molecular-weight molecules (LMW) (The LMW to HMW ratio (LHR) was statistically higher in the SIRS group compared to the non-SIRS group (p< 0.001). Canine SIRS may induce renal tubular damage. In SIRS dogs, proteinuria can be an early indicator of kidney damage, and an increase in tubular damage could indicate a negative prognostic factor in dogs with proteinuria.
