Professor Gyeongsang National University Jinjusi, Kyongsang-namdo, Republic of Korea
Abstract:
Background: The effect of acidemia on blood coagulation remains inadequately understood in veterinary medicine.
Objectives: To assess the effect of in vitro acidification of canine whole blood on coagulation, and to investigate whether acidemia-induced coagulopathy could be reversed by reversing acidemia.
Methods: Citrated whole blood samples were taken from six healthy Beagle dogs and categorized based on pH adjustment into: neutral, weak acidemia (WA), strong acidemia (SA), and reversal from SA. Then prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen concentration, conventional thromboelastography (TEG) parameters, and velocity curve (V-curve) variables of TEG were assessed.
Results: The PT, aPTT, and most of TEG parameters showed significant coagulopathy in the SA group when compared to neutral group, with additional significant changes in reaction time (R), clot kinetic (K), maximum amplitude (MA), split point (SP), elasticity (E), thrombodynamic potential index (TPI), and coagulation index (CI) between the SA and WA groups. Among V-curve variables, the maximum rate of thrombus generation (MRTG) and total thrombus generation (TG) were significantly inhibited in the SA group compared to the neutral group, with significant differences in the time to maximum rate of thrombus generation (TMRTG) between the WA and SA groups. In the reverse group, aPTT, R, K, α-angle, MRTG, TMRTG, SP, TPI, and CI exhibited significant recovery compared to the SA group.
Conclusions: In vitro induction of acidemia in canine whole blood leads to impairment of coagulation profiles, and pH correction can reverse most acidemia-induced coagulopathy.
