Global Diagnostics Medical Affairs Platform Lead Zoetis Parsippany, NJ, United States
Abstract:
Background: In-clinic analysis of warm, fresh urine provides rapid results of urine sediment findings. A simple, standardized preparation method would aid in performing urine sediments in-clinic. Hypothesis/
Objectives: The Vetscan Imagyst® preparation method (VSI-prep) will recover similar numbers of red blood cells, white blood cells, struvite crystals, calcium oxalate dihydrate crystals, squamous epithelial cells, other epithelial cells, hyaline casts, non-hyaline casts and rod and cocci bacteria as determined by manual microscopic examination by ACVP-boarded clinical pathologists (ACVP-CPs) as compared to reference lab preparation method (RL-prep).Animals: No animals were used in this study. Urine samples included a mix of client-owned dogs and cats undergoing urinalysis for any reason submitted to Zoetis Reference Laboratories. Some samples were artificially created by spiking donor urine with necessary elements (e.g., RBC from whole blood). A total of 213 paired urine samples consisting of 116 canine (54.5%) and 97 feline (45.5%) were evaluated.
Methods: Two 1mL aliquots were made from each urine sample, processed via VSI-prep or RL-prep, and blindly evaluated via manual microscopy by the same 2 ACVP-CPs. RL-prep served as the gold standard for comparison. ACVP-CPs recorded results for urine elements as an average of 10, 40X fields. Percent semi-quantification class agreement was calculated between methods and evaluated for each ACVP-CP. Species were combined for analysis.
Results: For elements, aggregate % agreement ranged between 76.6-88.9% (Table 1). Individual % agreements varied (Table 1). Conclusions and Clinical Importance: VSI-prep recovered similar numbers of urine sediment elements in canine and feline urine sediment samples as RL-prep.
